Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Evidence of anti-inflammatory and anti-ulcer properties of aerial parts of Centaurea tougourensis Boiss and Reut.

Mohamed Sabri Bensaad^1,2, Saliha Dassamiour² , Leila Hambaba², Mohamed Amine Kahoul³, Mohammed Benhoula⁴

¹Laboratoire de Physio-Toxicologie, Pathologie Cellulaires et Moleculaires-Biomolecules (LPTPCMB), Departement de Biologie des Organismes; ²Laboratoire de Biotechnologie des Molécules Bioactives et de la Physiopathologie Cellulaire (LBMBPC), Département de Microbiologie et de Biochimie, Faculte des Sciences de la Nature et de la Vie, Université de Batna-2; ³Laboratoire sciences des aliments (LSA), Institut des Sciences Vétérinaires et Agronomiques, Université de Batna-1, Batna ; 4Laboratoire de Microbiologie Appliquée (LMA), Département de Microbiologie, Faculté des Sciences de la Nature et de la Vie, Université de Béjaia, Béjaia, Algeria.

For correspondence:- Saliha Dassamiour Email: s.dassamiour@univ-batna2.dz Tel:+213670286603

Accepted: 17 July 2021 Published: 31 August 2021

Citation: Bensaad MS, Dassamiour S, Hambaba L, Kahoul MA, Benhoula M. Evidence of anti-inflammatory and anti-ulcer properties of aerial parts of Centaurea tougourensis Boiss and Reut.. Trop J Pharm Res 2021; 20(8):1647-1654 doi: 10.4314/tjpr.v20i8.14

© 2021 The authors.
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Abstract

Purpose: To determine the anti-inflammatory and anti-ulcer properties of the aerial parts of Centaurea tougourensis Boiss. & Reut.

Methods: The effects of n-butanol (n-BuOH) extract of the aerial part of Centaurea tougourensis on carrageenan-induced paw edema and ethanol-induced gastric mucosal damage were determined at 2 doses (200 and 400 mg/kg, po) in a mouse model. For each test, the animals were randomly divided into negative and positive control groups, as well as extract-treated groups. The mice were observed for any sign of inflammation for a period of 24h.

Results: Reduction of paw edema by C. tougourensis extract was highly significant (p < 0.001) at a dose of 400 mg/kg 24 h after carrageenan injection, with 55.26 % inhibition, followed very closely by 53.15 % inhibition at the dose of 200 mg/kg; indomethacin group showed an inhibition of 60 %. Histological examination supported the inhibition results. A significant reduction in inflammation by the extract at a dose of 400 mg/kg was also observed. No sign of ulcer was observed with C. tougourensis at the two doses (200 and 400 mg/kg). The total polyphenol content of the n-BuOH extract was 85.44 ?g gallic acid equivalent/mg of extract. Tannins were the most abundant fraction (51.87 ?g tannic acid equivalent/mg of extract), followed by flavonoids (25.55 ?g quercetin equivalent/mg of extract).

Conclusion: The results indicate that C. tougourensis may have potential beneficial effects in the treatment of diseases associated with inflammation and pain, besides its protective effect on the gastrointestinal tract.

Keywords: Anti-inflammatory, Anti-ulcer, Centaurea tougourensis, Flavonoids, Phenolics, Tannin